TISIGNER

Unleash the power of synthetic biology

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Bhandari, B.K., Lim, C.S., Gardner, P.P., (2021) TISIGNER.com: web services for improving recombinant protein production. Nucleic Acids Research. DOI:10.1093/nar/gkab175.
Our open source, industry-leading platform offers unparalleled speed and accuracy for gene optimisation.
TIsigner Translation Initiation coding region designer
TIsigner optimises the translation initiation coding region of a gene of interest to improve recombinant protein production in Escherichia coli, Saccharomyces cerevisiae and Mus musculus. In contrast to existing software, TIsigner suggests minimum numbers of synonymous codon changes to the input sequence. This enables a standard PCR cloning for the optimised sequence.
DOI: 10.1371/journal.pcbi.1009461
SoDoPE Soluble Domain for Protein Expression
SoDoPE enables users to navigate a protein sequence and its domains for predicting and maximising solubility. SoDoPE is linked with TIsigner, and therefore empowers protein biochemists to design sequences for tuning both protein expression and solubility for a gene of interest.
DOI: 10.1093/bioinformatics/btaa578
Leveraging 98,146 real-world experimental data points, our algorithms withstand the world's most stringent tests.
Highly accurate prediction of signal peptides from eukaryotic toxins and fungi.
Razor Signal peptide detector
Razor is built for detecting signal peptides in eukaryotes. Furthermore, we also detect whether the signal peptide is harboured by a toxin. Razor can also be used to check for signal peptides from fungi. Both toxin and fungi prediction is done using a novel approach of utilising the residues at the signal peptide itself.
DOI: 10.1101/2020.11.30.405613
Scalable prediction of protein-protein interactions (PPI) and interaction types.
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