General FAQ
Help and documentation for the TISIGNER suite
Help
TISIGNER is a suite of tools designed to improve outcomes of recombinant protein expression experiments.
If you have a nucleotide sequence, we recommend starting with SoDoPE to explore the solubility profile along the sequence. This is because some domains within a sequence may exhibit higher solubility compared to the full sequence.
By identifying these regions using SoDoPE, you can then optimize the mRNA for those specific areas with higher solubility using our TISIGNER mRNA optimization tool.
The Demo Mode button allows you to explore TISIGNER functionality without submitting requests to the live server. When enabled, sample data is loaded so you can try out our features safely. Toast notifications will appear to confirm that Demo Mode is active and show when demo results are loaded.
| Tool | Purpose | Status | FAQ |
|---|---|---|---|
| TIsigner | Checking and optimising protein expression | Running | Open FAQ |
| SoDoPE | Checking and optimising protein solubility | Running | Open FAQ |
| Razor | Detecting eukaryotic signal peptides. Additionally detects toxic proteins and fungal origin. | Retired | View GitHub |
| LazyPair | Predicting Protein-Protein Interactions | Retired | View GitHub |
Compatibility
| OS | Version | Chromium | Firefox |
|---|---|---|---|
| GNU/Linux | Ubuntu 26.04 LTS | 139.0.7214.0 | 150.0.2 |
| MacOS | 15.4.1 (24E263) | 139.0.7214.0 | 150.0.2 |
| Windows | 11 (25H2) | 139.0.7214.0 | 150.0.2 |
| Android/Linux | 15 | 147.0.7727.56 | 150.0.2 |
Citation
If you are using this webservice, please cite the following NAR paper for the webservice in addition to the citation for the specific tool.
TISIGNER.com: web services for improving recombinant protein production.
Bhandari BK, Lim CS, Gardner PP
Nucleic Acids Research (2021)
DOI: 10.1093/nar/gkab175Analysis of 11,430 recombinant protein production experiments reveals that protein yield is tunable by synonymous codon changes.
Bhandari BK, Lim CS, Remus DM, Chen A, Dolleweerd C, Gardner PP
PLOS Computational Biology (2021)
DOI: 10.1371/journal.pcbi.1009461Solubility-Weighted Index: Fast and Accurate Prediction of Protein Solubility.
Bhandari BK, Gardner PP, Lim CS
Bioinformatics (2020)
DOI: 10.1093/bioinformatics/btaa578Razor: annotation of signal peptides from toxins.
Bhandari BK, Gardner PP, Lim CS
bioRxiv (2021)
DOI: 10.1101/2020.11.30.405613LazyPair: scalable prediction of protein-protein interactions and interaction types.
Lim CS, Bhandari BK, Gardner PP
bioRxiv (2021)
DOI: 10.1101/2022.02.21.481370